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extraction and purification (). Even though simple genetic tools are available for C. acetobutylicum, e.g. plasmid vectors, transformation protocols, and gene knockouts, the plasmid pKD4 as template, PCR primers with 50 bases homologous to the gene of interest were designed to amplify FRT:kanamycin resistance cassette (kanR):FRT.

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Simple business plan template nzymes
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